| Registro completo |
| Provedor de dados: |
Biol. Res.
|
| País: |
Chile
|
| Título: |
Characterization of mitochondrial iron uptake in HepG2 cells
|
| Autores: |
MAZARIEGOS,DORA INÉS
CABANTCHIK,Z. IOAV
CASSELS,BRUCE K
NÚÑEZ,MARCO TULIO
|
| Data: |
2006-01-01
|
| Ano: |
2006
|
| Palavras-chave: |
Mitochondria
Iron homeostasis
RPA
HepG2 cells
|
| Resumo: |
There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content.
|
| Tipo: |
Journal article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024
|
| Editor: |
Sociedad de Biología de Chile
|
| Formato: |
text/html
|
| Fonte: |
Biological Research v.39 n.1 2006
|
|
